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This study was carried out to obtain the basic data for an artificial cultivation of Phellinus sp.. The optimum conditions for the mycelial growth on the different sawdusts (Quercus aliena, Morus alba and Alnus japonica) substrate of an isolated Phellinus sp. were optimized by response surface methodology. The ratio of rice bran addition to sawdust and the suitable moisture content for the mycelial growth in the all sawdust media were about 30% (w/w) and 65∼70% (w/v), respectively. The initial pHs for the mycelial growth of Quercus aliena and Morus alba were in the range of pH 5∼6, whereas Alnus japonica was obtained at pH 6. The optimum temperature for the mycelial growth was about 25∼30℃, depending on the different kinds of wood substrates. From the response surface analysis, the values of independent variables of Quercus aliena at stationary points were determined to be 31.01% (w/w) of rice bran, pH of 5.31 and 69.03% (w/v) of moisture content, and the expected value of mycelial growth was about 8.32 ㎝. Both the ratio of rice bran addition to sawdust (X₁) and moisture content (X₃) were effective to the mycelial growth. In the case of Morus alba, the ratio of rice bran addition to sawdust, initial pH and moisture content at the stationary points were 28.77% (w/w), 5.28 and 69.8 (w/v), respectively, and the expected mycelial growth of 7.60 ㎝ was obtained. Stationary points for the mycelial growth in the sawdust media of Alnus japonica were 28.74% (w/w) of rice bran, pH of 6.04 and 66.96% (w/v) of moisture content, and the expected values of mycelial growth was about 5.38 ㎝. Based on the above results, there was correlations between the mycelial growth and independent variables, and the effect of rice bran (X₃) and initial pH (X₂) for the mycelial growth were higher than the moisture content (X₃). The optimum species of sawdust media for the mycelial growth of Phellinus sp. was in the order of Quercus aliena $gt; Morus alba $gt; Alnus japonica.
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